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Filament rigidity and connectivity tune the deformation modes of active biopolymer networks

Supporting Information

  • Download Supporting Information (PDF)
  • Download Movie_S01 (MP4) - Time-lapse images of TMR-actin (red) and Alexa-642 myosin (white) in sample with no actin cross-linker. Time stamp is in minutes:seconds format and 0:00 indicates the time of the maximal density of myosin puncta.
  • Download Movie_S02 (MP4) - Time-lapse images of TMR-actin (red) and Alexa-642 myosin (white) in sample containing fascin (1:10 fascin:actin monomer) and filamin (1:500 filamin:actin monomer). Time stamp is in minutes:seconds format and 0:00 indicates the time of the maximal density of myosin puncta.
  • Download Movie_S03 (MP4) - Time-lapse images of TMR-actin (Left) and corresponding distribution of Mmin/Mmax (Right, colors indicating time) in network with no cross-linker. Each Mmin/Mmax distribution curve is cumulative over a 20-frame window (time between frames = 1 s), and the actin image displayed for each curve occurs in the middle (10th frame) of this window.
  • Download Movie_S04 (MP4) - Time-lapse images of TMR-actin (Left) and corresponding distribution of Mmin/Mmax (Right, colors indicating time) in sample containing fascin (1:10 fascin:actin monomer) and filamin (1:500 filamin:actin monomer). Each Mmin/Mmax distribution curve is cumulative over a 20-frame window (time between frames = 1 s), and the actin image displayed for each curve occurs in the middle (10th frame) of this window.
  • Download Movie_S05 (MP4) - Simulation images of semiflexible filaments (red) and myosin (white), where Lp=12.5?μm and ρxl=1?μm?2. Time delay between frames is 1 s.
  • Download Movie_S06 (MP4) - Simulation images of rigid filaments (red) and myosin (white) where Lp=250?μm and ρxl=1?μm?2. Time delay between frames is 1 s.
  • Download Movie_S07 (MP4) - Time-lapse images of TMR-actin (red) and Alexa-642 myosin (white) in sample containing fascin (1:10 fascin:actin monomer) and no filamin. Time stamp is in minutes:seconds format and 0:00 indicates the time of the maximal density of myosin puncta.
  • Download Movie_S08 (MP4) - Time-lapse images of TMR-actin (Left) and corresponding distribution of Mmin/Mmax (Right, colors indicating time) in sample containing fascin (1:10 fascin:actin monomer) and no filamin. Each Mmin/Mmax distribution curve is cumulative over a 20-frame window (time between frames = 1 s), and the actin image displayed for each curve occurs in the middle (10th frame) of this window.

Online Impact

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